![]() ![]() Arrangement of 30S heterogeneous nuclear ribonucleoprotein on polyoma virus late nuclear transcripts. Identification and characterization of the packaging proteins of core 40S hnRNP particles. Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. The spliceosome: design principles of a dynamic RNP machine. Expansion of the eukaryotic proteome by alternative splicing. The ability of high-resolution iCLIP data to provide insights into the mechanism of this regulation holds promise for studies of other higher-order ribonucleoprotein complexes. Integration of transcriptome-wide iCLIP data and alternative splicing profiles into an 'RNA map' indicates how the positioning of hnRNP particles determines their effect on the inclusion of alternative exons. hnRNP particles assemble on both introns and exons but remain generally excluded from splice sites. iCLIP data show that hnRNP C recognizes uridine tracts with a defined long-range spacing consistent with hnRNP particle organization. ![]() Here, we developed individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to study the role of hnRNP C in splicing regulation. Despite their abundance, however, it remained unclear whether these particles control pre-mRNA processing. In the nucleus of eukaryotic cells, nascent transcripts are associated with heterogeneous nuclear ribonucleoprotein (hnRNP) particles that are nucleated by hnRNP C. ![]()
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